SphI
SphI cuts DNA at GCATGC, leaving a 4-nucleotide 3′ overhang (CATG). Its 6 bp site occurs about once every 4,096 bp of random sequence.
Recognition & cut site
GCATG^C
Properties
- Recognition site
- GCATGC
- Cut site
- GCATG^C
- Ends produced
- 3′ overhang
- Overhang
- CATG (4 nt)
- Site length
- 6 bp
- Cuts on average
- 1 / 4,096 bp
- Palindromic
- Yes
- Cuts within site
- Yes (type II)
Sequence checker
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