NspI

NspI cuts DNA at RCATGY, leaving a 4-nucleotide 3′ overhang (CATG). Its 6 bp site occurs about once every 1,024 bp of random sequence.

Recognition & cut site

RCATG^Y

Properties

Recognition site
RCATGY
Cut site
RCATG^Y
Ends produced
3′ overhang
Overhang
CATG (4 nt)
Site length
6 bp
Cuts on average
1 / 1,024 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

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Related enzymes

Isoschizomers

same site, same cut
  • BstNSI
  • NspHI
  • PauAI
  • PunAII
  • XceI

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang
  • nlaiii
  • sphi
  • BbuI
  • FaeI
  • Hin1II
  • HpyCH4I
  • Hsp92II
  • PaeI
  • PfaAIII
  • RspLKI
  • SpaHI