NlaIII

NlaIII cuts DNA at CATG, leaving a 4-nucleotide 3′ overhang (CATG). Its 4 bp site occurs about once every 256 bp of random sequence.

Recognition & cut site

CATG^

Properties

Recognition site
CATG
Cut site
CATG^
Ends produced
3′ overhang
Overhang
CATG (4 nt)
Site length
4 bp
Cuts on average
1 / 256 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut
  • FaeI
  • Hin1II
  • HpyCH4I
  • Hsp92II

Neoschizomers

same site, different cut position
  • CviAII
  • FatI

Compatible ends

different site, matching overhang
  • nspi
  • sphi
  • BbuI
  • BstNSI
  • NspHI
  • PaeI
  • PauAI
  • PfaAIII
  • PunAII
  • RspLKI
  • SpaHI
  • XceI