XmaI
XmaI cuts CCCGGG after the first C, leaving a 4-nucleotide 5′ overhang (CCGG); its neoschizomer SmaI cuts the same sequence centrally for blunt ends.
Recognition & cut site
C^CCGGG
Properties
- Recognition site
- CCCGGG
- Cut site
- C^CCGGG
- Ends produced
- 5′ overhang
- Overhang
- CCGG (4 nt)
- Site length
- 6 bp
- Cuts on average
- 1 / 4,096 bp
- Palindromic
- Yes
- Cuts within site
- Yes (type II)
Sequence checker
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Related enzymes
Isoschizomers
same site, same cut- AhyI
- Cfr9I
- EaeAI
- EclRI
- Pac25I
- PspAI
- TspMI
- XcyI
- XmaCI
Neoschizomers
same site, different cut position- smai
- CfrJ4I
- PaeBI
- PspALI