BspEI

BspEI cuts DNA at TCCGGA, leaving a 4-nucleotide 5′ overhang (CCGG). Its 6 bp site occurs about once every 4,096 bp of random sequence.

Recognition & cut site

T^CCGGA

Properties

Recognition site: TCCGGA
Cut site: T^CCGGA
Ends produced: 5′ overhang
Overhang: CCGG (4 nt)
Site length: 6 bp
Cuts on average: 1 / 4,096 bp
Palindromic: Yes
Cuts within site: Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut

AccIII
Aor13HI
BbvAIII
BlfI
BseAI
BsiMI
Bsp13I
BspMII
Bsu23I
CauB3I
Kpn2I
MroI
PinBII
PtaI

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang