Transformation Efficiency Calculator

Calculate transformation efficiency in CFU per µg from colonies, DNA and volumes, with a benchmark of typical preps.

DNA transformed
ng/µL
µL

Concentration × volume gives the DNA transformed

Plating
µL
µL
Interpretation
Transformation efficiency
—cfu/µg
Interpretation
—
Benchmark — log scale vs. typical prep grades
low / poorchemically competentelectrocompetentlibrary10⁴10⁶10⁸10¹⁰10¹²
cfu/µg = colonies × (total volume ÷ volume plated) × dilution ÷ µg DNA