ScaI

ScaI cuts DNA at AGTACT, leaving blunt ends. Its 6 bp site occurs about once every 4,096 bp of random sequence.

Recognition & cut site

AGT^ACT

Properties

Recognition site
AGTACT
Cut site
AGT^ACT
Ends produced
blunt
Overhang
—
Site length
6 bp
Cuts on average
1 / 4,096 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

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Related enzymes

Isoschizomers

same site, same cut
  • Acc113I
  • AssI
  • BmcAI
  • DpaI
  • Eco255I
  • RflFII
  • ZrmI

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang

None known.