SacI

SacI cuts DNA at GAGCTC, leaving a 4-nucleotide 3′ overhang (AGCT). Its 6 bp site occurs about once every 4,096 bp of random sequence.

Recognition & cut site

GAGCT^C

Properties

Recognition site
GAGCTC
Cut site
GAGCT^C
Ends produced
3′ overhang
Overhang
AGCT (4 nt)
Site length
6 bp
Cuts on average
1 / 4,096 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut
  • Psp124BI
  • SstI

Neoschizomers

same site, different cut position
  • BpuAmI
  • Ecl136II
  • Eco53kI
  • EcoICRI
  • MxaI

Compatible ends

different site, matching overhang

None known.