RsaI

RsaI cuts DNA at GTAC, leaving blunt ends. Its 4 bp site occurs about once every 256 bp of random sequence.

Recognition and cut site

Blunt ends; no overhang

5′GTAC3′
3′CATG5′

Properties

Recognition site
GTAC
Cut site
GT^AC
Ends produced
blunt
Overhang
—
Site length
4 bp
Cuts on average
1 / 256 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut
  • AfaI
  • HpyBI
  • PlaAII

Neoschizomers

same site, different cut position
  • Csp6I
  • CviQI
  • CviRII
  • PabI
  • RsaNI

Compatible ends

different site, matching overhang

None known.