PacI

PacI cuts DNA at TTAATTAA, leaving a 2-nucleotide 3′ overhang (AT). Its 8 bp site occurs about once every 65,536 bp of random sequence.

Recognition & cut site

TTAAT^TAA

Properties

Recognition site
TTAATTAA
Cut site
TTAAT^TAA
Ends produced
3′ overhang
Overhang
AT (2 nt)
Site length
8 bp
Cuts on average
1 / 65,536 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut

None known.

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang
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