NotI
NotI cuts DNA at GCGGCCGC, leaving a 4-nucleotide 5′ overhang (GGCC). Its 8 bp site occurs about once every 65,536 bp of random sequence.
Recognition & cut site
GC^GGCCGC
Properties
- Recognition site
- GCGGCCGC
- Cut site
- GC^GGCCGC
- Ends produced
- 5′ overhang
- Overhang
- GGCC (4 nt)
- Site length
- 8 bp
- Cuts on average
- 1 / 65,536 bp
- Palindromic
- Yes
- Cuts within site
- Yes (type II)
Sequence checker
Runs in your browser. Nothing is uploaded.
Related enzymes
Isoschizomers
same site, same cut- CciNI
- CsiBI
- CspBI
- MchAI
Neoschizomers
same site, different cut positionNone known.
Compatible ends
different site, matching overhang- eagi
- AaaI
- AcoI
- Bfi89I
- BseX3I
- Bsp120I
- BstZI
- CfrI
- EaeI
- EclXI
- Eco52I
- EcoHK31I
- PspOMI
- SenPT16I
- XmaIII