NcoI

NcoI cuts DNA at CCATGG, leaving a 4-nucleotide 5′ overhang (CATG). Its 6 bp site occurs about once every 4,096 bp of random sequence.

Recognition & cut site

C^CATGG

Properties

Recognition site
CCATGG
Cut site
C^CATGG
Ends produced
5′ overhang
Overhang
CATG (4 nt)
Site length
6 bp
Cuts on average
1 / 4,096 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut
  • Bsp19I

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang
  • BspHI
  • BspLU11I
  • CciI
  • FatI
  • PagI
  • PciI
  • PscI
  • RcaI
  • RspXI