MseI
MseI cuts DNA at TTAA, leaving a 2-nucleotide 5′ overhang (TA). Its 4 bp site occurs about once every 256 bp of random sequence.
Recognition & cut site
T^TAA
Properties
- Recognition site
- TTAA
- Cut site
- T^TAA
- Ends produced
- 5′ overhang
- Overhang
- TA (2 nt)
- Site length
- 4 bp
- Cuts on average
- 1 / 256 bp
- Palindromic
- Yes
- Cuts within site
- Yes (type II)
Sequence checker
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