KpnI

KpnI cuts DNA at GGTACC, leaving a 4-nucleotide 3′ overhang (GTAC). Its 6 bp site occurs about once every 4,096 bp of random sequence.

Recognition & cut site

GGTAC^C

Properties

Recognition site
GGTACC
Cut site
GGTAC^C
Ends produced
3′ overhang
Overhang
GTAC (4 nt)
Site length
6 bp
Cuts on average
1 / 4,096 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut

None known.

Neoschizomers

same site, different cut position
  • Acc65I
  • AhaB8I
  • Asp718I
  • SthI

Compatible ends

different site, matching overhang

None known.