HpaI

HpaI cuts DNA at GTTAAC, leaving blunt ends. Its 6 bp site occurs about once every 4,096 bp of random sequence.

Recognition & cut site

GTT^AAC

Properties

Recognition site
GTTAAC
Cut site
GTT^AAC
Ends produced
blunt
Overhang
Site length
6 bp
Cuts on average
1 / 4,096 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut
  • BstEZ359I
  • BstHPI
  • KspAI
  • SsrI

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang

None known.