HinfI

HinfI cuts DNA at GANTC, leaving a 3-nucleotide 5′ overhang that varies with the N in its site.

Recognition and cut site

3-nt 5′ overhang; sequence set by the flanking DNA

5′GANTC3′
3′CTNAG5′

Properties

Recognition site
GANTC
Cut site
G^ANTC
Ends produced
5′ overhang
Overhang
variable (3 nt)
Site length
5 bp
Cuts on average
1 / 256 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

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Related enzymes

Isoschizomers

same site, same cut
  • CviBI
  • FnuAI
  • HhaII
  • SscL1I

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang

None known.