HhaI

HhaI cuts DNA at GCGC, leaving a 2-nucleotide 3′ overhang (CG). Its 4 bp site occurs about once every 256 bp of random sequence.

Recognition & cut site

GCG^C

Properties

Recognition site
GCGC
Cut site
GCG^C
Ends produced
3′ overhang
Overhang
CG (2 nt)
Site length
4 bp
Cuts on average
1 / 256 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut
  • AspLEI
  • BspLAI
  • BstHHI
  • CfoI
  • FnuDIII

Neoschizomers

same site, different cut position
  • Hin6I
  • HinP1I
  • HsoI
  • HspAI
  • SciNI

Compatible ends

different site, matching overhang

None known.