DpnI

DpnI cuts GATC only when the adenine is Dam-methylated, which is why it removes the plasmid template after site-directed mutagenesis.

Recognition & cut site

GA^TC

Properties

Recognition site
GATC
Cut site
GA^TC
Ends produced
blunt
Overhang
—
Site length
4 bp
Cuts on average
1 / 256 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

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Related enzymes

Isoschizomers

same site, same cut
  • CfuI
  • MalI

Neoschizomers

same site, different cut position
  • dpnii
  • mboi
  • sau3ai
  • AspMDI
  • Bce243I
  • Bfi57I
  • BfuCI
  • Bme12I
  • BscFI
  • Bsp105I
  • Bsp143I
  • Bsp2095I
  • Bsp67I
  • BspAI
  • BspFI
  • BspJI
  • BspKT6I
  • BssMI
  • Bst19II
  • BstENII
  • BstKTI
  • BstMBI
  • BtkII
  • CacI
  • CcyI
  • ChaI
  • CpfI
  • CviAI
  • FnuCI
  • FnuEI
  • HacI
  • Kzo9I
  • LlaAI
  • MgoI
  • MkrAI
  • NdeII
  • NlaII
  • NmeCI
  • NphI
  • RalF40I
  • SauMI
  • SsiAI
  • SsiBI
  • Sth368I

Compatible ends

different site, matching overhang

None known.