DdeI
DdeI cuts DNA at CTNAG, leaving a 3-nucleotide 5′ overhang that varies with the N in its site.
Recognition & cut site
C^TNAG
Properties
- Recognition site
- CTNAG
- Cut site
- C^TNAG
- Ends produced
- 5′ overhang
- Overhang
- variable (3 nt)
- Site length
- 5 bp
- Cuts on average
- 1 / 256 bp
- Palindromic
- Yes
- Cuts within site
- Yes (type II)
Sequence checker
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Related enzymes
Isoschizomers
same site, same cut- BstDEI
- BstJZ301I
- HpyF3I
Neoschizomers
same site, different cut positionNone known.
Compatible ends
different site, matching overhangNone known.