ClaI

ClaI cuts DNA at ATCGAT, leaving a 2-nucleotide 5′ overhang (CG). Its 6 bp site occurs about once every 4,096 bp of random sequence.

Recognition & cut site

AT^CGAT

Properties

Recognition site: ATCGAT
Cut site: AT^CGAT
Ends produced: 5′ overhang
Overhang: CG (2 nt)
Site length: 6 bp
Cuts on average: 1 / 4,096 bp
Palindromic: Yes
Cuts within site: Yes (type II)

Sequence checker

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Related enzymes

Isoschizomers

same site, same cut

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang