BstXI

BstXI cuts DNA at CCANNNNNNTGG, leaving a 4-nucleotide 3′ overhang that varies with the N in its site.

Recognition and cut site

4-nt 3′ overhang; sequence set by the flanking DNA

5′CCANNNNNNTGG3′
3′GGTNNNNNNACC5′

Properties

Recognition site
CCANNNNNNTGG
Cut site
CCANNNNN^NTGG
Ends produced
3′ overhang
Overhang
variable (4 nt)
Site length
12 bp
Cuts on average
1 / 4,096 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut
  • BstHZ55I

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang

None known.