BsmFI

BsmFI recognizes GGGAC and cuts at a fixed distance downstream, leaving a 4-nucleotide overhang set by the flanking DNA.

Recognition & cut site

GGGAC(10/14)

Properties

Recognition site
GGGAC
Cut site
GGGAC(10/14)
Ends produced
5′ overhang
Overhang
variable (4 nt)
Site length
5 bp
Cuts on average
1 / 1,024 bp
Palindromic
No
Cuts within site
No (type IIS)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut
  • BpuSI
  • BslFI
  • BspLU11III
  • BstOZ616I
  • FaqI

Neoschizomers

same site, different cut position
  • FinI

Compatible ends

different site, matching overhang

None known.