BsaI

BsaI recognizes GGTCTC and cuts downstream of it, so the 4-nucleotide overhang is chosen by design — the basis of Golden Gate assembly.

Recognition & cut site

GGTCTC(1/5)

Properties

Recognition site
GGTCTC
Cut site
GGTCTC(1/5)
Ends produced
5′ overhang
Overhang
variable (4 nt)
Site length
6 bp
Cuts on average
1 / 4,096 bp
Palindromic
No
Cuts within site
No (type IIS)

Sequence checker

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Related enzymes

Isoschizomers

same site, same cut
  • Bli736I
  • Bso31I
  • BspTNI
  • Eco31I
  • EcoA4I
  • EcoO44I

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang

None known.