BfuAI
BfuAI recognizes ACCTGC and cuts at a fixed distance downstream, leaving a 4-nucleotide overhang set by the flanking DNA.
Recognition & cut site
ACCTGC(4/8)
Properties
- Recognition site
- ACCTGC
- Cut site
- ACCTGC(4/8)
- Ends produced
- 5′ overhang
- Overhang
- variable (4 nt)
- Site length
- 6 bp
- Cuts on average
- 1 / 4,096 bp
- Palindromic
- No
- Cuts within site
- No (type IIS)
Sequence checker
Runs in your browser. Nothing is uploaded.
Related enzymes
Isoschizomers
same site, same cut- Acc36I
- BspMI
- BveI
Neoschizomers
same site, different cut positionNone known.
Compatible ends
different site, matching overhangNone known.