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1. Pick a single yeast colony and grow in 4 ml YPD medium overnight at 30°C.
2. Transfer 200 µl to 5 ml sterile ddH2O.
3. Centrifuge briefly to pellet the cells.
4. Resuspend cells in 2.5 ml SPM (0.3% Potassium acetate and 0.02% D(+)-Raffinose Pentahydrate).
5. Incubate at 25-30 °C for 2-5 days.
6. Check presence of asci with phase contrast microscopy and dissect.
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