Storage of baculovirus stocks

1. Harvest the supernatant contianing the baculovirus. Centrifuge down cells and remove supernatnant containing the virus to fresh tubes.

2. For short term storage, store at 4 C in dark.

3. For long term storage, aliquot, and freeze at - 80 C.

4. If a serum-free medium is used, add BSA to final concentration of 0.1-1%. If desired, virus can also be filtered through a 0.2 mm, low-protein binding filter.