2. Submerge the gel in the Commassie blue stain solution in a box with lid (e.g, an empty pipet tip box). Microwave on high for 1 min, then shake 10-20 min.

Coomassie blue stain solution can be reused for serveral times.

3. Destaining in several changes of de-staining solution until protein bands are clear. Microwave on high for 1 min and shake until the bands emerge clearly from the background. Put a small sponge in the destaining solution to absorb coomassie blue dye particles.

4. Gel can be photographed under white light, stored in the destaining solution, or dried.