Edited by Chang Zhu
1. Volatile 125I2 may be
produced. Work under a chemical hood. Proper
protection is also needed when working with 125I.
2. Dilute 2 mg of protein to 20 ml in
50 mM sodium phosphate (pH 7.4), 0.15 M NaCl.
3. Add 50 ml 0.5 M sodium phosphate (pH 7.0).
4. Add 1 mCi of Na125I.
5. Add 5 ml chloramine T (0.5 mg/ml in
50 mM sodium phosphate (pH 7.4), 0.15 M NaCl). Prepare chloramine solution
immediately before use.
6. Incubate for 15 seconds to 5 minutes.
7. Stop the reaction by adding 10 ml
Na2SO3 or
Na2S3O5 (5 mg/ml).
8. Separate by gel filtration.
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