Electroporation --- parameters for different cells
ChangBioscience.com
The parameters in the table below may be used as a starting point for
optimization. 4 mm cuvettes are used for animal cells and 1 mm for others.
|
V
(v) |
C (mF) |
Q
(c) |
R
(W
) |
V/D (kv/cm) |
|
BHK-21 |
400 |
960 |
0.4 |
|
1 |
|
Cos-7 |
350 |
500 |
0.2 |
|
0.9 |
|
ES |
200 |
500 |
0.1 |
|
0.5 |
|
HL-60 |
350 |
960 |
0.3 |
|
0.9 |
|
Jurkat |
250 |
960 |
0.2 |
|
0.6 |
|
PC12 |
200 |
800 |
0.2 |
|
0.5 |
|
U936 |
350 |
960 |
0.3 |
|
0.9 |
|
|
|
|
|
|
|
Aspergillus nidulans |
500 |
25 |
|
400 |
5 |
|
E. Coli |
1700 |
25000 |
|
200 |
17 |
|
Neurospora crassa |
150 |
25000 |
|
600 |
1.5 |
|
Yeast |
900 |
50 |
|
100 |
9 |
|
|
