|
1. Place fixed tissues in the tissue processor.
2. Process using the following or similar program.
|
Station |
Solution |
Concentration |
Time(min) |
Temp(°C) |
|
1 |
Ethanol |
70% |
30 |
|
|
2 |
Ethanol |
80% |
30 |
|
|
3 |
Ethanol |
95% |
45 |
|
|
4 |
Ethanol |
95% |
45 |
|
|
5 |
Ethanol |
100% |
45 |
|
|
6 |
Ethanol |
100% |
45 |
|
|
7 |
Xylenes |
100% |
45 |
|
|
8 |
Xylenes |
100% |
45 |
|
|
9 |
Paraffin |
|
30 |
60 |
|
10 |
Paraffin |
|
30 |
60 |
|
11 |
Paraffin |
|
30 |
60 |
|
12 |
Paraffin |
|
30 |
60 |
3. Embed in paraffin and block.
4. Cut sections at 5-8 ml thickness.
5. Paraffin blocks and sections may be stored at room temperature or below.
| 