PCR cleanup (Microcon)

1. Place a Microcon-30 in a microcentrifuge tube. Add PCR reaction products.

2. Add 450 ml H2O to the Microcon-30.

3. Centrifuge at 12,000 rpm for ~ 10 minutes or until the retained volume is approximately 50 ml.

4. Discard flow-through.

5. Repeat the wash (step 2-4) 2 more times.

6. Invert microcon in a new tube and elute by spinning 1 minute.

Check Microcon Cutoff for chosen an appropriate Microcon filter.