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1. Mix equal mole of both complementary oligos in a 1.5 ml
eppendorf tube:
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oligo 1 (0.1-1 mg)
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2 ml
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oligo 2 (0.1-1 mg)
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2 ml
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10X Annealing Buffer (100 mM Tris-HCl pH8.0, 500 mM NaCl, 10 mM EDTA)
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2 ml
|
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ddH2O
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14 ml
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2. Place tube in a heatblock filled with water at 90 °C for 5 minutes.
3. Take the heatblock out or turn the power off, allow it to cool slowly
to room temperature.
4. Place on ice or 4 °C until ready to use.
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