Virtualab Protocols (Chang Bioscience)
1. Most introns start with GU and end with AG. A small fraction of introns
start with AT and ends with AC.
2. The branch site is located 20-50 bases upstream of the acceptor
site.
5'-splice site
(donor)
|
Branch site
|
3'-splice site
(acceptor)
|
|
A/C A G
|
G U Pu A G U
C U Pu A Py
Py rich
N C A G
|
G
|
Pu = A or G, Py = C or U, and N = Pu or Py.
|
