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Inhibitor
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Specificity
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Stock
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Aprotinin
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Serine protease inhibitor. Inhibits plasmin, trypsin, and chymotrypsin.
Does not inhibit thrombin or Factor X.
|
10 mg/ml in water. Store at -20 °C. Stable for at least 1 week at 4 °C.
|
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EDTA
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Inhibits metalloproteases.
|
0.5 M in water (pH8-9). Store at 4 °C. Stable at 4 °C.
|
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EGTA
|
Ca2+-dependent proteases.
|
200 mg/ml in 1N NaOH. Store at 4 °C. Stable at 4 °C.
|
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Leupeptin
|
inhibits serine and cysteine proteases such as trpsin, papain,
and cathepsin B.
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1 mg/ml in water. Store at -20 °C. Stable at least 1 week at 4 °C.
|
|
Pepstatin
|
inhibits aspartic acid proteases such as pepsin, renin, cathepsin D,
chymosin.
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1 mg/ml in methanol. Store at -20 °C. Stable at least 1 week at 4 °C.
|
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PMSF (Phenylmethylsulfonyl fluoride)
|
inhibits serine and cysteine proteases such as chymotrypsin, trypsin, thrombin,
and papain.
|
10 mg/ml in isopropanol or ethanol. Store at -20 °C. Stable in isopropanol, unstable in water.
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