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Stock solution A
0.2 M monobasic sodium phosphate, monohydrate (27.6g/L)
Stock solution B
0.2 M dibasic sodium phosphate (28.4 g/L).
Mixing
an appropriate volume (ml) of A and B as shown in the table
below and diluting to a total volume of 200 ml, to make a 0.1 M phosphate
buffer of the required pH at room temperature. To make a 1 M phosphate
buffer starting with 2 M A and B stocks.
0.2 M monobasic sodium phosphate |
0.2 M dibasic sodium phosphate | pH
| |
92.0 | 8.0 | 5.8
| |
90.0 | 10.0 | 5.9
| |
87.7 | 12.3 | 6.0
| |
85.5 | 15.0 | 6.1
| |
81.5 | 19.5 | 6.2
| |
77.5 | 22.5 | 6.3
| |
73.5 | 26.5 | 6.4
| |
68.5 | 31.5 | 6.5
| |
62.5 | 37.5 | 6.6
| |
56.5 | 43.5 | 6.7
| |
51.0 | 49.0 | 6.8
| |
45.0 | 55.0 | 6.9
| |
39.0 | 61.0 | 7.0
| |
33.0 | 67.0 | 7.1
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28.0 | 72.0 | 7.2
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23.0 | 77.0 | 7.3
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19.0 | 81.0 | 7.4
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16.0 | 84.0 | 7.5
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13.0 | 87.0 | 7.6
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10.5 | 89.5 | 7.7
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8.5 | 91.5 | 7.8
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