|
|
Gel |
Bromophenol blue |
Xylene cyanol FF |
Orange G |
|
DNA agarose |
|
|
|
|
0.8%-2.5% |
100-300 bp |
800-4,000 bp |
30-50 bp |
|
Non-denaturing polyacrylamide gel |
|
|
|
|
3.50% |
100 bp |
460 bp |
|
|
5.00% |
65 bp |
260 bp |
|
|
8.00% |
45 bp |
160 bp |
|
|
12.00% |
20 bp |
70 bp |
|
|
15.00% |
15 bp |
60 bp |
|
|
20.00% |
12 bp |
45 bp |
|
|
Denaturing polyacrylamide gel |
|
|
|
|
5.00% |
35 nucleotides |
140 nucleotides |
|
|
6.00% |
26 nucleotides |
106 nucleotides |
|
|
8.00% |
19 nucleotides |
75 nucleotides |
|
|
10.00% |
12 nucleotides |
55 nucleotides |
|
|
20.00% |
8 nucleotides |
28 nucleotides |
|
|
