l HindIII

l HindIII-EcoRI

l EcoRI

f X174 HaeII

100 bp ladder

1 kb ladder

23,130

21,226

21,226

1,353

2,072

12,216

9,416

5,148

7,421

1,078

1,500

11,198

6,557

4,973

5,804

872

1,400

10,180

4,361

4,268

5,643

603

1,300

9,162

2,322

3,530

4,878

310

1,200

8,144

2,027

2,027

3,530

281

1,100

7,126

564

1,904

271

1,000

6,108

125

1,584

234

900

5,090

1,375

194

800

4,072

947

118

700

3,054

831

72

600

2,036

564

500

1,636

125

400

1,018

300

517

200

506

100

396

344

298

220

201

154

134

75