Chicken Polyclonal Antibody Production Schedule Edited by Chang Zhu Chickens produce mostly IgY in eggs (~50 mg antibody per egg). Week 0 Collect an egg (pre-immune egg). Immunize with 200 µg/chicken IM/SQ antigen in Complete Freund's Adjuvant . Week 1 Immunize with 100 µg/chicken IM/SQ antigen in Incomplete Freund's Adjuvant (IFA). Week 2 Immunize with 100 µg/chicken IM/SQ antigen in PBS. Week 3 Immunize with 100 µg/chicken IM/SQ antigen in PBS. Week 7 Immunize with 100 µg/chicken IM/SQ antigen in PBS. Week 4-13 Collect eggs.