Science Gateway

Tools: Bacteria Transformation Efficiency Calculator

Transformation efficiency (transformants/g) is calculated as follows:
# colonies on plate/ng of DNA plated X 1000 ng/g
 
Enter the concentration (in μg/μl) of
the DNA used to transform:
μg/μl
 
Enter the volume (in μl) of
DNA added to the transformation mix:
μl
 
Enter the volume (in μl) of
the total transformation reaction*:
μl
 
Enter the volume (in μl) actually plated
from the transformation mix (for a single plate):
μl
 
Enter the number of colonies or
transformants from the plate above:

Transformation Efficiency:
Transformants / μg DNA or
Transformants / ng of DNA
 
*This calculation assumes that no futher dilutions of the transformation reaction are made after DNA, competent cells, and media are added.