Transformation Efficiency Calculator

Calculate transformation efficiency in CFU per µg from colonies, DNA and volumes, with a benchmark of typical preps.

DNA transformed

Concentration

ng/µL

Volume added

µL

Concentration × volume gives the DNA transformed

Plating

Total reaction volume

µL

Volume plated

µL

Extra dilution (fold)

Colonies counted

Interpretation

Cell type

Transformation efficiency

— cfu/µg

Interpretation

—

Benchmarkcfu/µg, log scale

PoorChemicalElectroLibrary

10⁴10⁶10⁸10¹⁰10¹²

Formula

cfu/µg = colonies × (total volume ÷ volume plated) × dilution ÷ µg DNA