Tools: PCR Master Mix Calculator

Input:

Results:

Total Nr. of PCR Reactions Volume per reaction: µl Total volume µl DNA µl PCR buffer µl Primer 1 µl Primer 2 µl dNTP mix µl Enzyme µl MgCl2 µl µl µl

µl DNA (Total) Total volume in master mix: µl H2O µl PCR buffer µl Primer 1 µl Primer 2 µl dNTP mix µl MgCl2 µl µl µl µl Enzyme µl Total Volume µl/reaction without DNA