NheI

NheI cuts DNA at GCTAGC, leaving a 4-nucleotide 5′ overhang (CTAG). Its 6 bp site occurs about once every 4,096 bp of random sequence.

Recognition and cut site

4-nt 5′ overhang; CTAG

5′GCTAGC3′
3′CGATCG5′

Properties

Recognition site
GCTAGC
Cut site
G^CTAGC
Ends produced
5′ overhang
Overhang
CTAG (4 nt)
Site length
6 bp
Cuts on average
1 / 4,096 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

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Related enzymes

Isoschizomers

same site, same cut
  • AsuNHI
  • LlaG2I
  • PstNHI

Neoschizomers

same site, different cut position
  • AceII
  • BmtI
  • BspOI

Compatible ends

different site, matching overhang