MluI
MluI cuts DNA at ACGCGT, leaving a 4-nucleotide 5′ overhang (CGCG). Its 6 bp site occurs about once every 4,096 bp of random sequence.
Recognition and cut site
4-nt 5′ overhang; CGCG
5′ACGCGT3′
3′TGCGCA5′
Properties
- Recognition site
- ACGCGT
- Cut site
- A^CGCGT
- Ends produced
- 5′ overhang
- Overhang
- CGCG (4 nt)
- Site length
- 6 bp
- Cuts on average
- 1 / 4,096 bp
- Palindromic
- Yes
- Cuts within site
- Yes (type II)
Sequence checker
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Related enzymes
Isoschizomers
same site, same cut- Bbi24I
Neoschizomers
same site, different cut positionNone known.
Compatible ends
different site, matching overhang- asci
- BsePI
- BssHII
- BstBZ153I
- PalAI
- PauI
- PteI
- SelI
- SgsI