An anti-CD151 monoclonal antibody strongly inhibited alpha3-dependent migration of beta-glucan-stimulated neutrophils under agarose. These results will appear in an article by Yauch et al., Mol.Biol.Cell, in 1998.
Yauch et al. recently discovered that CD151 association with the alpha3beta1 integrin is unusually stable, specific, and highly stoichiometric. Furthermore, CD151 appears to provide the major link between PI 4-kinase and the alpha3beta1 integrin. These results will appear in Mol.Biol.Cell, in 1998.
Yauch RL; Berditchevski F; Harler MB; Reichner J; Hemler ME. Highly stoichiometric, stable, and specific association of integrin alpha3beta1 with CD151 provides a major link to phosphatidylinositol 4-kinase, and may regulate cell migration. Mol. Biol. Cell 1998 9:2751 PubMed
We have analyzed CD151/integrin associations using both an adherent cell line (HT1080) and a suspension cell line (K562), and we have prepared cell lysates using either 1% Brij 96, 1% Brij 99, or 1% octylglucoside. In all cases we have been able to either co-immunoprecipitate or re-immunoprecipitate alpha3 and alpha6 integrins, but not alpha2 or alpha5 integrins. In addition, from CD151 complexes, we have successfully immunoblotted only alpha3 and alpha6 integrins, but not alpha2 or alpha5 integrins. While of course different results may be obtained on other cell lines, we conclude at present that alpha5 and alpha2 integrins are not readily observed to associate with CD151 (Yauch et al., 1998, Mol.Biol.Cell, in press, and unpublished)
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