PROW: CD66c

PROW and IWHLDA present the GUIDE on:
CD66c
Authors: Keith Skubitz; Peter Jantscheff; Motomu Kuroki; Amy Skubitz
Reviewer:
Diverse coauthors in lieu of reviewer
Link to additional info in FORUM [GUIDE BAR]

ALTERNATE NAMES FOR CD66c

NCA
NCA-50/90

MAJOR LINKS FOR CD66c

NCBI LocusLink Record: 4680
Mendelian Inheritance in Man (OMIM): 163980
SwissProt annotated protein record: P40199

FUNCTION BIOCHEMICAL ACTIVITY OF CD66c - Unclear, see ligands

CELLULAR FUNCTION OF CD66c

Unclear, but capable of homophilic adhesion, heterophilic adhesion, E-selectin binding, galectin binding, type I fimbriae binding, and transmembrane signaling
Capable of activating neutrophils

DISEASE RELEVANCE OF CD66c AND FUNCTION OF CD66c IN INTACT ANIMAL

Unclear, see cellular function

STRUCTURE MOLECULAR FAMILY FOR CD66c

Families in which CD66c is a member
- CD66c-->carcinoembryonic antigen-->immunoglobulin supergene family

MOLECULAR STRUCTURE OF CD66c

Leader 34 aa
Extracellular 284 aa
A 24 aa hydrophobic domain replaced with GPI
2 Ig C2 domains and 1 IgG-V like domain

MOLECULAR MASS OF CD66c

CELL TYPE	MW UNREDUCED	MW REDUCED	Comment
Various cells		90 kDa

POST-TRANSCRIPTIONAL MODIFICATION OF CD66c

GPI-linked, no splice variants

POST-TRANSLATIONAL MODIFICATION OF CD66c

Heavily and variably N-glycosylated; (12 N-glycosylation sites)

MOLECULAR INTERACTIONS PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD66c - No information

SUBSTRATES FOR CD66c - No information

ENZYMES WHICH MODIFY CD66c - No information

LIGANDS FOR CD66c AND MOLECULES ASSOCIATED WITH CD66c Link to additional info in FORUM

MOLECULE	COMMENT
Unknown molecule on bacteria	Binds the carbohydrate on CD66c not the protein core
CD62E (E-selectin)	Binds the carbohydrate on CD66c not the protein core
Galectins	Binds the carbohydrate on CD66c not the protein core
CD66a	Binds the protein core of CD66c not the carbohydrate
CD66b	Binds the protein core of CD66c not the carbohydrate
CD66c	Binds the protein core of CD66c not the carbohydrate
CD66e	Binds the protein core of CD66c not the carbohydrate
src family kinases	Some src family kinases associate with CD66c in neutrophils

EXPRESSION MAIN CELLULAR EXPRESSION OF CD66c

Granulocytes and epithelial cells

AUTHOR'S ADDITIONAL INSIGHTS ON CD66c

CD66c may play an important role in sites of inflammation
The data are consistent with the hypothesis that CD66c plays a signaling role and regulates the adhesion activity of CD11/CD18 in neutrophils. While the details of the "activation signal" transmitted by CD66 antigens are not known, the finding of tyrosine kinase activity associated with CD66a, CD66b, and CD66c suggests that these kinase activities may be involved in signal transduction via CD66 family members

REAGENTS CD66c-SPECIFIC MABS NEWLY ASSIGNED AT SIXTH INTERNATIONAL WORKSHOP - See reference mAb

SELECTION OF OTHER CD66c-SPECIFIC REFERENCE MAB

NAME(Workshop IDs)	SOURCE or REFERENCE	COMMENT
9A6	J Johnson and Fritz Grunert,

SELECTED REFERENCES ON CD66c

REVIEWS

PRIMARY CITATIONS

1. Kleist S von,Chavanel G,Burtin P Identification of an antigen from normal human tissue that crossreacts with the carcinoembryonic antigen. Proc Natl Acad Sci U S A 1972 69:2492 PubMed

2. Thompson JA,Grunert F,Zimmermann W Carcinoembryonic antigen gene family: molecular biology and clinical perspectives. J Clin Lab Anal 1991 5:344 PubMed

3. Audette M,Buchegger F,Schreyer M,Mach JP Monoclonal antibody against carcinoembryonic antigen (CEA) identifies two new forms of crossreacting antigens of molecular weight 90,000 and 160,000 in normal granulocytes. Mol Immunol 1987 24:1177 PubMed

4. Tawaragi Y,Oikawa S,Matsuoka Y,Kosaki G,Nakazato H Primary structure of nonspecific crossreacting antigen (NCA), a member of carcinoembryonic antigen (CEA) gene family, deduced from cDNA sequence. Biochem Biophys Res Commun 1988 150:89 PubMed

5. Oikawa S,Inuzuka C,Kuroki M,Matsuoka Y,Kosaki G,Nakazato H Cell adhesion activity of non-specific cross-reacting antigen (NCA) and carcinoembryonic antigen (CEA) expressed on CHO cell surface: homophilic and heterophilic adhesion. Biochem Biophys Res Commun 1989 164:39 PubMed

6. Leusch HG,Hefta SA,Drzeniek Z,Hummel K,Markos-Pusztai Z,Wagener C Escherichia coli of human origin binds to carcinoembryonic antigen (CEA) and non-specific crossreacting antigen (NCA). FEBS Lett 1990 261:405 PubMed

7. Kuijpers TW,Hoogerwerf M,van der Laan LJ,Nagel G,van der Schoot CE,Grunert F,Roos D CD66 nonspecific cross-reacting antigens are involved in neutrophil adherence to cytokine-activated endothelial cells. J Cell Biol 1992 118:457 PubMed

8. Skubitz KM,Campbell KD,Ahmed K,Skubitz AP CD66 family members are associated with tyrosine kinase activity in human neutrophils. J Immunol 1995 155:5382 PubMed

9. Ohannesian DW,Lotan D,Thomas P,Jessup JM,Fukuda M,Gabius HJ,Lotan R Carcinoembryonic antigen and other glycoconjugates act as ligands for galectin-3 in human colon carcinoma cells. Cancer Res 1995 55:2191 PubMed

10. Skubitz KM,Campbell KD,Skubitz AP CD66a, CD66b, CD66c, and CD66d each independently stimulate neutrophils. J Leukoc Biol 1996 60:106 PubMed

WWW RESOURCES

* indicates ammended by reviewer, ** indicates added by reviewer

Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission

Modified 10/14/99 mpr@mail.nih.gov