• 4F2
• FRP-1
• RL-388

MAJOR LINKS FOR CD98  

• NCBI LocusLink Record: 4198
• Mendelian Inheritance in Man (OMIM): 158070
• SwissProt annotated protein record: P08195

• Potential amino acid transporter

CELLULAR FUNCTION OF CD98  

• Involved in the regulation of cellular activation

DISEASE RELEVANCE OF CD98 AND FUNCTION OF CD98 IN INTACT ANIMAL  

• Up-regulated on leukocytes in inflammatory lesions, and strongly expressed by neoplastic cells. However, expression is not hematopoietic specific

MOLECULAR STRUCTURE OF CD98  

• Disulfide-linked heterodimer
• Heavy chain: Comprised of external carboxy-terminus, internal leader sequence, single transmembrane domain, and two possible membrane-associated domains (5)
• Heavy chain is weakly homologous to certain amino acid transporters (4)

MOLECULAR MASS OF CD98  

POST-TRANSCRIPTIONAL MODIFICATION OF CD98   - Unknown

POST-TRANSLATIONAL MODIFICATION OF CD98  

• Glycosylated heavy chain, and a non-glycosylated light chain

• CD98 heavy chain expression involves protein kinase C and calcium-mediated activation pathways
• G+C rich housekeeping promoter which contains four Sp1 binding sites

SUBSTRATES FOR CD98  

ENZYMES WHICH MODIFY CD98   - No information

LIGANDS FOR CD98 AND MOLECULES ASSOCIATED WITH CD98  

• Broad reactivity on activated and transformed cells, not hematopoietic specific, and found at lower levels on quiescent cells

• Anti-CD98 mAbs have been shown to induce syncitia formation (2) and inhibit T cell proliferation to nominal antigens, lectins and superantigen. This has been shown to occur by triggering CD98 on monocytes

SELECTION OF OTHER CD98-SPECIFIC REFERENCE MAB  

REVIEWS

PRIMARY CITATIONS

1. Haynes BF,Hemler ME,Mann DL,Eisenbarth GS,Shelhamer J,Mostowski HS,Thomas CA,Strominger JL,Fauci AS Characterization of a monoclonal antibody (4F2) that binds to human monocytes and to a subset of activated lymphocytes. J Immunol 1981 126:1409 PubMed

2. Ohgimoto S,Tabata N,Suga S,Nishio M,Ohta H,Tsurudome M,Komada H,Kawano M,Watanabe N,Ito Y Molecular characterization of fusion regulatory protein-1 (FRP-1) that induces multinucleated giant cell formation of monocytes and HIV gp160- mediated cell fusion. FRP-1 and 4F2/CD98 are identical molecules. J Immunol 1995 155:3585 PubMed

3. Freidman AW,Diaz LA Jr,Moore S,Schaller J,Fox DA The human 4F2 antigen: evidence for cryptic and noncryptic epitopes and for a role of 4F2 in human T lymphocyte activation. Cell Immunol 1994 154:253 PubMed

4. Bertran J,Magagnin S,Werner A,Markovich D,Biber J,Testar X,Zorzano A,Kuhn LC,Palacin M,Murer H Stimulation of system y(+)-like amino acid transport by the heavy chain of human 4F2 surface antigen in Xenopus laevis oocytes. Proc Natl Acad Sci U S A 1992 89:5606 PubMed

5. Lumadue JA,Glick AB,Ruddle FH Cloning, sequence analysis, and expression of the large subunit of the human lymphocyte activation antigen 4F2. Proc Natl Acad Sci U S A 1987 84:9204 PubMed

6. Leiden JM,Yang LH,Morle GD,June CH,Lindsten T,Thompson CB,Karpinski B The 4F2 heavy chain gene: a molecular model of inducible gene expression in human T cells. J Autoimmun 1989 2:67 PubMed

7. Karpinski BA,Yang LH,Cacheris P,Morle GD,Leiden JM The first intron of the 4F2 heavy-chain gene contains a transcriptional enhancer element that binds multiple nuclear proteins. Mol Cell Biol 1989 9:2588 PubMed

8. Boumsell, L, Leucocyte Typing V: White Cell Differentiation Antigens, S.F. Schlossman, et al, Eds. (Oxford, Oxford, 1995), p. 280.

9. Wells RG,Lee WS,Kanai Y,Leiden JM,Hediger MA The 4F2 antigen heavy chain induces uptake of neutral and dibasic amino acids in Xenopus oocytes. J Biol Chem 1992 267:15285 PubMed

10. Palacin M A new family of proteins (rBAT and 4F2hc) involved in cationic and zwitterionic amino acid transport: a tale of two proteins in search of a transport function. J Exp Biol 1994 196:123 PubMed

11. Bron C,Rousseaux M,Spiazzi AL,MacDonald HR Structural homology between the human 4F2 antigen and a murine cell surface glycoprotein associated with lymphocyte activation. J Immunol 1986 137:397 PubMed

12. Lindsten T,June CH,Thompson CB,Leiden JM Regulation of 4F2 heavy-chain gene expression during normal human T- cell activation can be mediated by multiple distinct molecular mechanisms. Mol Cell Biol 1988 8:3820 PubMed

13. Gottesdiener KM,Karpinski BA,Lindsten T,Strominger JL,Jones NH,Thompson CB,Leiden JM Isolation and structural characterization of the human 4F2 heavy-chain gene, an inducible gene involved in T-lymphocyte activation. Mol Cell Biol 1988 8:3809 PubMed

14. Parmacek MS,Karpinski BA,Gottesdiener KM,Thompson CB,Leiden JM Structure, expression and regulation of the murine 4F2 heavy chain. Nucleic Acids Res 1989 17:1915 PubMed

15. Francke U,Foellmer BE,Haynes BF Chromosome mapping of human cell surface molecules: monoclonal anti- human lymphocyte antibodies 4F2, A3D8, and A1G3 define antigens controlled by different regions of chromosome 11. Somatic Cell Genet 1983 9:333 PubMed

WWW RESOURCES

Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission

Modified 10/14/99   mpr@mail.nih.gov