• Rh-associated protein*
• gp42
• integrin-associated protein, IAP
• neurophilin
• ovarian carcinoma antigen 3, OA3
• MEM-133
• CDw149

MAJOR LINKS FOR CD47  

• NCBI LocusLink Record: 961
• Mendelian Inheritance in Man (OMIM): 601028
• SwissProt annotated protein record: Q08722

CELLULAR FUNCTION OF CD47  

• Adhesion molecule
• Thrombospondin receptor

DISEASE RELEVANCE OF CD47 AND FUNCTION OF CD47 IN INTACT ANIMAL  

• CD47 knockout mice showed a defect in host defense
• Granulocytes from CD47 knockout mice were deficient in [beta]3 integrin-dependent ligand binding, cell migration and activation

• Families in which CD47 is a member
  • CD47-->immunoglobulin supergene family

MOLECULAR STRUCTURE OF CD47  

• An extracellular immunoglobulin (IgV)-like domain (~120 amino acids)
• Five membrane spanning segments (~152 amino acids)
• A short hydrophobic intracytoplasmic tail (~30 amino acids)

MOLECULAR MASS OF CD47  

POST-TRANSCRIPTIONAL MODIFICATION OF CD47  

• Four isoforms of mRNA derived by alternative splicing at the intercytoplasmic carboxyl termini

POST-TRANSLATIONAL MODIFICATION OF CD47  

• Five potential N-glycosylation sites in the extracellular domain, three are glycosylated in erythrocytes

SUBSTRATES FOR CD47   - No information

ENZYMES WHICH MODIFY CD47   - No information

LIGANDS FOR CD47 AND MOLECULES ASSOCIATED WITH CD47  

• Broad tissue distribution including haemopoietic cells, epthelial and endothelial cells, fibroblasts and other tissue cells (strong expression in brain), mesenchymal cell, tumour cell lines.

• Ligands which interact with CD47 are not found
• The role of CD47 in cell activation needs to be further studied

SELECTION OF OTHER CD47-SPECIFIC REFERENCE MAB  

REVIEWS

1. Anstee, D. J. and Spring, F. A. In Leucocyte Typing V (ed. Schlossan et al.), pp. 233-4. Oxford University Press. Oxford (1995).

PRIMARY CITATIONS

2. Brown E,Hooper L,Ho T,Gresham H Integrin-associated protein: a 50-kD plasma membrane antigen physically and functionally associated with integrins. J Cell Biol 1990 111:2785 PubMed

3. Campbell IG,Freemont PS,Foulkes W,Trowsdale J An ovarian tumor marker with homology to vaccinia virus contains an IgV- like region and multiple transmembrane domains. Cancer Res 1992 52:5416 PubMed

4. Gao AG,Lindberg FP,Finn MB,Blystone SD,Brown EJ,Frazier WA Integrin-associated protein is a receptor for the C-terminal domain of thrombospondin. J Biol Chem 1996 271:21 PubMed

5. Lindberg FP,Bullard DC,Caver TE,Gresham HD,Beaudet AL,Brown EJ Decreased resistance to bacterial infection and granulocyte defects in IAP-deficient mice. Science 1996 274:795 PubMed

6. Lindberg FP,Lublin DM,Telen MJ,Veile RA,Miller YE,Donis-Keller H,Brown EJ Rh-related antigen CD47 is the signal-transducer integrin-associated protein. J Biol Chem 1994 269:1567 PubMed

7. Mawby WJ,Holmes CH,Anstee DJ,Spring FA,Tanner MJ Isolation and characterization of CD47 glycoprotein: a multispanning membrane protein which is the same as integrin-associated protein (IAP) and the ovarian tumour marker OA3. Biochem J 1994 304:525 PubMed

8. Reinhold MI,Lindberg FP,Plas D,Reynolds S,Peters MG,Brown EJ In vivo expression of alternatively spliced forms of integrin- associated protein (CD47). J Cell Sci 1995 108:3419 PubMed

WWW RESOURCES

Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission

Modified 10/14/99   mpr@mail.nih.gov