• Thy-1

MAJOR LINKS FOR CD90  

• NCBI LocusLink Record: 7070
• Mendelian Inheritance in Man (OMIM): 188230
• SwissProt annotated protein record: P04216

CELLULAR FUNCTION OF CD90  

• In mouse may contribute to lymphocyte costimulation
• May contribute to inhibition of proliferation differentiation of hematopoietic stem cells and neuron memory formation in the CNS

DISEASE RELEVANCE OF CD90 AND FUNCTION OF CD90 IN INTACT ANIMAL  

• CD90 mAb expression inhibits neurite outgrowth on mature astrocytes [Tiveron MC et al. 1992]
• CD90 mAb block memory formation in chicks in passive avoidance tasks [Feng H et al. 1993]
• CD90 knockout mice showed decreased in vivo formation of long term potentiation in the hippocampal dentate gyrus [Nosten-Bertrand M et al. 1996]
• Cross-linking of CD90 at the surface of CD90 positive hematopoietic progenitors inhibits production of primitive hematopoietic colonies [Mayani and Lansdorp 1994]**
• CD90 mAbs very useful means of identifying CD34+hematopoietic precursor cells in vitro [Craig et al. 1993] and in vivo*
• CD34+CD90+ cells include hematopoietic stem cells that can serve as autologous grafts to replace the bone marrow in patients with malignancies [Tsukamoto AS et al. 1994]*

• Families in which CD90 is a member
  • CD90-->immunoglobulin supergene family

MOLECULAR STRUCTURE OF CD90  

• Single Ig domain (112 amino acids) inserted into membrane via GPI anchor

MOLECULAR MASS OF CD90  

POST-TRANSCRIPTIONAL MODIFICATION OF CD90   - No information

POST-TRANSLATIONAL MODIFICATION OF CD90  

• Three N-linked glycosylation sites
• Composition of carbohydrates varies between tissues of same animal and within same tissue at different stages of differentiation

SUBSTRATES FOR CD90   - None

ENZYMES WHICH MODIFY CD90   - None

LIGANDS FOR CD90 AND MOLECULES ASSOCIATED WITH CD90  

• Expressed by hematopoietic stem cells and neurons in all species studied
• Highly expressed in connective tissue and various fibroblast and stromal cell lines
• Expressed on all thymocytes and peripheral T cells in mice, but in humans expressed only on small % fetal thymocytes, 10-40% of CD34+ cells in bone marrow, and <1% of CD3+CD4+ lymphocytes in peripheral circulation
• Also expressed by human lymph node HEV endothelium but not other endothelia
• Lastly, expressed by limited number of lymphoblastoid and leukemic cell lines

• Two allelic forms exist in humans but polymorphic site is in third intron and so does not give rise to different allelic proteins
• Both ligands and function of CD90 in the human immune system remain
• unidentified

SELECTION OF OTHER CD90-SPECIFIC REFERENCE MAB  

REVIEWS

1. Cell surface Thy-1 (ed. A. F. Reif and M. Schlesinger). 1989. Marcel Dekker, Inc. New York.

PRIMARY CITATIONS

2. Craig W,Kay R,Cutler RL,Lansdorp PM Expression of Thy-1 on human hematopoietic progenitor cells. J Exp Med 1993 177:1331 PubMed

3. Feng H,Zhao WQ,Bernard CC,Ng KT,Sedman G Purified antichick Thy-1 IgG abolishes intermediate and long-term memory. Physiol Behav 1993 53:215 PubMed

4. Mayani H,Lansdorp PM Thy-1 expression is linked to functional properties of primitive hematopoietic progenitor cells from human umbilical cord blood. Blood 1994 83:2410 ** PubMed

5. Nosten-Bertrand M,Errington ML,Murphy KP,Tokugawa Y,Barboni E,Kozlova E,Michalovich D,Morris RG,Silver J,Stewart CL,Bliss TV,Morris RJ Normal spatial learning despite regional inhibition of LTP in mice lacking Thy-1. Nature 1996 379:826 PubMed

6. Tiveron MC,Barboni E,Pliego Rivero FB,Gormley AM,Seeley PJ,Grosveld F,Morris R Selective inhibition of neurite outgrowth on mature astrocytes by Thy-1 glycoprotein. Nature 1992 355:745 PubMed

7. Tsukamoto AS,Reading C,Carella A,Frassoni F,Gorin C,LaPorte J,Negrin R,Blume K,Cunningham I,Deisseroth A,et al Biological characterization of stem cell present in mobilized peripheral blood of CML patients. Bone Marrow Transplant 1994 14:S25 PubMed

WWW RESOURCES

Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission

Modified 10/14/99   mpr@mail.nih.gov