7.001 Hypertextbook
Recombinant DNA Chapter Directory

1. Cloning into plasmids
2. Southerns, Westerns, and Northerns: Molecular Cloning Techniques
   • Theory: Complementarity and Hybridization
     
   • Basic Definitions
     
   • Overview
     
   • Gel electrophoresis
     
   • Transfer to Solid Support
     
   • Blocking
     
   • Preparing the Probe
     
   • Hybridization
     
   • Washing
     
   • Detecting of Probe-Target Hybrids
     
   • Summary of Blots
   • Cloning a Gene By Hybridization
     
   • Solving problems
     • Southern Blot Problem
     • Northern Blot Problem
     • Western Blot Problem
3. The Polymerase Chain Reaction (PCR)
4. DNA Fingerprinting
   • DNA Fingerprinting in Human Health and Society
   • An Interview with DNA Forensics Authority Dr. Bruce Weir
   • Use of DNA in Identification: A talk by Eric Lander
5. Practice problems