Science Gateway

Protocols: ES Cell Culture - Gene Targeting - Protocols

source: Bradley Laboratory Homepage

Tissue Culture Protocols

  • General ES Cell Protocols/Starting Out With ES Cells

     

  • Recipes for Tissue Culture Selective Drug Stocks

     

  • Components and Supplementation of Various Culture Media

     

  • Electroporation into ES cells

     

  • Analysis of ES Cell Clones by Mini-Southern

     

  • Picking ES Cell Colonies and Transferring Them to 24-Well Culture Dishes

     

  • Picking ES Cell Colonies and Transferring Them to 96-Well PLATES

     

  • Use of the Hemacytometer for the Determination of Cell Numbers

     

  • beta-Galactosidase Color Assay for Cultured Cells

     

  • Isolation of Primary Fibroblasts from Mouse Embryos

     

  • Preparation of Feeder Layers And SNL Stocks

     

  • Preparing 48-Well Plate Feeders

     

  • Frozen Feeders

     

  • Freezing and Thawing Cultured Cells

     

  • Feeder Preparation by Gamma Irration

     

  • Freezing Embryonic Stem (ES) Cell Clones in 96-Well Plates

     

  • Preparation of RNA from Cultured Cells

     

    Other Protocols

  • Colony Hybridization

     

  • Genomic DNA:Restriction Enzyme Digests, Agarose Gel Electrophoresis, and Southern Transfer (Blotting)

     

  • Transfer of DNA to GeneScreen Plus Using the Bio-Dot SF Apparatus

    •