Training and regulation
Only qualified personnel with proper training and registration may handle radioactive material and
Document usage and disposal.
Document contamination survey.
Know the isotope
Isotopes used in biological sciences.
Turn on a survey meter (e.g., Geiger-Mueller counter for beta-emitter)
Wear lab coat and gloves
Use proper shields
-Bench top shield screen
-Microtube block rack
-Beta waste safe box
-Lead shield and lead foil
Keep a distance
Be ready, minimize exposure time
Working in designated area
Cover bench and working area with assay mats
Place paper towels in easy to reach area
Keep only necessary materials and equipment in the working area
Never centrifuge a radioactive sample without a cap or cover
When boiling samples in microcentrifuge tubes, lock with cap lock
Don't generate gaseous radioactive waste except for 14C and 3H
Don't use 35S in a thermocycler
Before using a survey meter, make sure the survey meter is working properly.
Check for battery and calibrate regularly.
Monitor with Geiger-Mueller counter for high energy beta emitters (e.g., 32P)
Monitor with portable thin crystal NaI scintillation counter (e.g., 125I).
Monitor with liquid scintillation counter.
Monitor for exposure
Wear a radiation dosimeter.
Check for contamination
Clean contaminated area with soap and water or radiacwash decontaminant